Curriculum Vita


EDUCATION:

1980-1982.  South Oklahoma City Junior College, Oklahoma City, Oklahoma.

B.S.  1982-1986.  Cum Laude.  Department of Biology, Central State University, Edmond, Oklahoma. 

1987-1989.  Graduate student.  Oklahoma State University, Stillwater, Oklahoma.

Ph.D. 1989-1994.  Department of Biochemistry and Molecular Biology, LSU Medical Center-Shreveport, Shreveport, Louisiana.

EXPERIENCE:

Graduate Student.  1989 - 1994.  Thesis advisor: Dr. Peter Zuber, Department of
Biochemistry and Molecular Biology, LSU Medical Center-Shreveport, Shreveport, Louisiana.  Research topic:  Genetic analysis of the nutritional source-dependent regulation of a sporulation gene, spoVG, in the Gram-positive soil bacterium, Bacillus subtilis.

Postdoctoral Fellow.   1994 -1998.  In the laboratory of Dr. James B. Rand, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma. Research interest:  Genetic analysis of the cholinergic nervous system in the soil nematode, Caenorhabditis elegans.

Assistant Professor of Biology    1998 - 2000.  University of Central Oklahoma. 
Research interest:  Genetic analysis of the cholinergic nervous system in the soil nematode, Caenorhabditis elegans.

Teaching Experience:  General Biology 1214, General Biology Laboratory 1214L, Introduction to Microbiology and Lab 2314, Cell Biology 2203,Embryology and Lab 3104, Cell Physiology 4454/5454.

Adjunct Professor at the University of Oklahoma:  Taught Animal Development during the spring semester of 2001. 

Research Assistant Member.   2000-2002.  In the laboratory of Dr. James B. Rand,
Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma.

Research interest:  Genetic analysis of the cholinergic nervous system in the soil nematode, Caenorhabditis elegans.

Project Manager:  Oversight and supervision of five research technicians.

Self Employed.  2002-2004.  Owner/operator of Blue Fox Studios.
Specializing in educational video and audio production, curriculum development,    
graphics, and animation.  I have worked with the OMRF as a conslultant on the Telescience educational  project.  My role included content creation, script writing, graphics, animation and audio/video production.

Research Associate.  9/1/2003-3/31/2004.  In the laboratory of Dr. Tina Henkin, The Ohio State University, Columbus, Ohio. Research interest:  Regulation of lysine biosynthesis at the level of post-transcription initiation in the soil bacterium, Bacillus subtilis.

Temporary Assistant Professor of Biology.    8/1/04- 7/31/05.  Cameron University. 

Teaching Experience:  General Biology 1004, General Biology Laboratory 1004L.  Genetics 3014 and Genetics Laboratory 3014L

McNair Scholars Program Academic Coordinator.    Fall 2004-2007.  Cameron University.
Duties include counseling scholars, conducting and coordinating workshops, and coordinating summer research internships.

Assitant Professor of Biology.      8/1/05 – Present.  Cameron University.

Teaching Experience:  Online General Biology 1004,  Principles of Biology I 1634, Genetics 3014, Genetics Laboratory 3014L, Introduction to Microbiology 2124, Principles of Microbiology 3154 and Principles of Microbiology Laboratory 3154L.

AWARDS  AND HONORS:
Alpha Chi National Honor Society, 1985
Outstanding Biology Senior Award, 1986
Student Speaker Award, Wind River Conference on Genetic Exchange, 1990
National Research Service Award (NIH), 1995, 1997
Sigma Xi Research Society, UCO Chapter, 1998
OCAST Oklahoma Health Research (OHR) Grant, 1999
INBRE Summer Internship Mentor, 2006
INBRE Research Minigrant, 2006
INBRE Junior Investigator Award, 2006
INBRE Summer Internship Mentor, 2007

PUBLICATIONS:

Thesis:

Frisby, D. L.  1994.  Genetics of carbon and nitrogen source-dependent regulation of sporulation gene expression in Bacillus subtilis.  Ph.D. Thesis.  Louisiana State University Medical Center-Shreveport.  Shreveport, Louisiana.

Research Papers and Book Chapters:

Frisby, D. L., and P. Zuber.  1991.  Analysis of the upstream activating sequence and site of carbon and nitrogen source repression in the promoter of an early induced sporulation gene of Bacillus subtilis.  J. Bacteriol.  173:7557-7564.

Frisby, D. L., and P. Zuber.   1994.  Mutations in pts  cause catabolite resistant sporulation and altered regulation of spo0H  in Bacillus subtilis.  J. Bacteriol.  176:2587-2595

D'Souza, C., M. M. Nakano, D. L. Frisby, and P. Zuber.  1995.  Translation of the open reading frame encoded by comS, a gene of the srf operon, is necessary for the development of genetic competence, but not surfactin biosynthesis, in Bacillus subtilis. 
J. Bacteriol. 177:4144-4148

Rand, J. B., J. S. Duerr, and D. L. Frisby.  1998.  Neurogenetics of synaptic transmission in C. elegans. Adv. Pharmacol. 42: 940-944.

Rand, J. B., J. S. Duerr, and D. L. Frisby.  1998.  Using C. elegans to study vesicular transport.  Methods Enzymol.  296:529-547

Duerr, J. S., D. L. Frisby, J. D. Erickson, A. Duke, L. E. Eiden, and J. B. Rand.  1999.  The cat-1 gene of Caenorhabditis elegans encodes a vesicular monoamine transporter required for specific mono-amine-dependent behaviors.  J. Neurosci. 1:72-84

Rand, J. B., Duerr, J. S. and Frisby, D. L., 2000.  Neurogenetics of vesicular transporters in C. elegans.  FASEB J. 14:2414-2422.

Lickteig, K.M., Duerr, J.S., Frisby, D.L., Hall, D.H., Rand, J.B., and Miller, D.M., III, 2001. Regulation of neurotransmitter vesicles by the homeodomain protein UNC-4 and its transcriptional co-repressor UNC-37/Groucho in Caenorhabditis elegans cholinergic motor neurons. J. Neurosci. 21: 2001-2014.

Mullen, G.P., Mathews, E.A., Vu, M.H., Hunter, J.W., Frisby, D.L., Duke, A., Grundahl, K., Osborne, J.D., Crowell, J.A., and J.B. Rand.  2007.  Choline transport and de novo choline synthesis support acetylcholine biosynthesis in C. elegans cholinergic neruons.  Genetics.  In Press.

Frisby, D. L., Fields, S. P., Duerr, J. S., McManus, J. R., Grundahl, K. M., Duke, A., Crowell, J. A., Osborne, J., Saliba, D. L., and Rand, J. B., 2007.  Analysis of the cholinergic promoter region in C. elegans.  In Preparation.

Abstracts:

Frisby, D. L., and P. Zuber.  1990.  Mutational analysis of a stationary phase-induced promoter in Bacillus subtilis.  Presented at the 34th Annual Wind River Conference on Genetic Exchange.  Estes Park, Colorado.

Frisby, D. L., and P. Zuber.  1990.  Mutational analysis of a stationary phase-induced promoter in Bacillus subtilis:  evidence for carbon and nitrogen source control.  Presented at the 90th General Meeting of the American Society for Microbiology.  Dallas, Texas.

Frisby, D. L., and P. Zuber.  1991.  Mutational analysis of carbon and nitrogen source-dependent regulation of a stationary phase-induced promoter in Bacillus subtilis.  Presented at the Molecular Genetics of Bacteria and Phages meeting, Cold Spring Harbor Laboratory.  Cold Spring Harbor, New York.

Frisby, D. L., and P. Zuber.  1992.  Carbon and nitrogen source-dependent regulation of a sporulation gene in Bacillus subtilis.  Presented at the 11th Annual Spores Conference.  Marine Biological Laboratories, Woods Hole, Massachusetts.

Frisby, D. L., and P. Zuber.  1993.  Mutations in the pts  region of Bacillus subtilis  cause catabolite resistant sporulation and enhanced spo0H  expression.  Keystone Symposium on the Molecular Genetic Controls of Microbial Differentiation.  Keystone, Colorado.

Frisby, D. L., J. Duerr, J. McManus, and J. Rand.  1995.  Analysis of the upstream regulatory region of the cha-1/unc-17 locus.  Presented at the 10th International C. elegans Meeting.  University of Wisconsin-Madison.

Frisby, D. L., J. Duerr, J. McManus, and J. Rand.  1995.  Regulation of cholinergic function in Caenorhabditis elegans.  Presented at the 25th Annual Meeting of the Society for Neuroscience.  San Diego, California.

Cosby, W. M., D. L. Frisby, and P. Zuber.  1996.  Isolation and characterization of mutants of glucose-glutamine-dependent repression of late-expression genes in Bacillus subtilis.  Presented at the 96th General Meeting of the American Society for Microbiology.  New Orleans, Louisiana.

Cosby, W. M., D. L. Frisby, and P. Zuber.  1996.  Low growth-medium pH negatively affects spo0H-dependent gene expression in Bacillus subtilis.  Presented at the 12th International Spores Conference.  University of Wisconsin-Madison.

Frisby, D. L., J. McManus, J. Duerr, and J. Rand.  1996.  Regulation of cholinergic gene expression in Caenorhabditis elegans.  Presented at the Midwest Caenorhabditis elegans meeting.  Normal, Illinois.

Frisby, D. L., J. McManus, J. Duerr, and J. Rand.  1996.  Regulation of cholinergic gene expression in Caenorhabditis elegans.  Presented at the 26th Annual Meeting of the Society for Neuroscience.  Washington D.C.

Frisby, D. L., J. McManus, J. Duerr, and J. Rand.  1997.  Regulation of cholinergic function in Caenorhabditis elegans.  Presented at the 11th International C. elegans Meeting.  University of Wisconsin-Madison.

Duerr, J. S., D. L. Frisby, J. Gaskin, A. Duke, J. D. Erickson, L. E. Eiden, and J. B. Rand.  1997.  Colocalization of two vesicular transporters in identified neurons of Caenorhabditis elegans.  Mol. Bio. Cell 8:410a.

Frisby, D. L.,  K. M. Lickteig, J. S. Duerr, J. R. McManus, D. M. Miller III, and J. B. Rand.  1998.  Regulation of cholinergic expression in specific motor neurons of Caenorhabditis elegans through an OLF-1/EBF-type binding site.  Presented at the 28th Annual Meeting of the Society for Neuroscience. Abstr. 24:1338.

Lickteig, K. M., J. Duerr, D. Frisby, J. Rand, and D. M. Miller III.  1998.  Regulation of vesicular proteins by UNC-4 and UNC-37.  Presented at the East Coast Caenorhabditis elegans meeting.  Abstr. 8.

Lickteig, K. M., D. Frisby, J. Duerr, D. M. and Miller III, and J. Rand.  1998.  Transcriptional control of cholinergic motor neuron differentiation: unc-3 regulates unc-17 and cha-1.  Presented at the East Coast Caenorhabditis elegans meeting.  Abstr. 116.

Lickteig, K. M., J. Duerr, D. Frisby, J. Rand, and D. M. Miller III.  1998.  Regulation of vesicular proteins by UNC-4 and UNC-37.  Presented at the Midwest Caenorhabditis elegans meeting.  Abstr. 69.

Lickteig, K. M., D. Frisby, J. Duerr, D. M. Miller III, and J. Rand.  1998.  Transcriptional control of cholinergic motor neuron differentiation: unc-3 regulates unc-17 and cha-1.  Presented at the Midwest Caenorhabditis elegans meeting.  Abstr. 70.

Duerr, J., J. Gaskin, D. Frisby, and J. Rand.  1999.  Development of Neurotransmitter Phenotypes in the VC Motor Neurons.  Presented at the 12th International Caenorhabditis elegans Meeting.  University of Wisconsin-Madison. Abstr. 283.

Lickteig, K. M., J. Duerr, D. Frisby, J. Rand, and D. M. Miller.  1999.  Regulation of vesicular proteins by UNC-4 and UNC-37.  Presented at the 12th International Caenorhabditis elegans Meeting.  University of Wisconsin-Madison. Abstr. 530.

Lickteig, K. M., D. Frisby, J. Duerr, D. M. Miller, and J. Rand.  1999.  UNC-3 regulates cholinergic motor neuron differentiation.  Presented at the 12th International Caenorhabditis elegans Meeting.  University of Wisconsin-Madison. Abstr. 531.

Lickteig, K. M., J. S. Duerr, D. L. Frisby, D. H. Hall, J. B. Rand, and D. M. Miller. 2000.  The UNC-4 homeoprotein and its transcriptional co-repressor UNC-37/Groucho regulate neurotransmitter vesicles in cholinergic motor neurons.  Presented at the West Coast Caenorhabditis elegans meeting.  Abstr. 86.

Frisby, D., T. Crowell, J. McManus, J. Rand.  2001.  Evidence for a cha-1-specific promoter in Caenorhabditis elegans.  Presented at the 13th International Caenorhabditis elegans Meeting abstract 671

Rand, J., Crowell, T., Duke, A., Frisby, D., Grundahl, K., Mcmanus, J.  2003.  The choline transporter is required for cholinergic function under conditions of high neurotransmitter demand.  Presented at the 2003 International Worm Meeting abstract 594.

Rand, J., Mcmanus, J., Grundahl, K., Frisby, D., Fields, S., Duke, A.  2003.  The rip-1 gene encodes a set of presynaptic proteins similar to vertebrate RIM and Piccolo  Presented at the 2003 International Worm Meeting abstract 595.

Mullen G., Mathews, E, Vu, M., Fields, S., Duke, A., Frisby, D., Rand, J.  2006. The Caenorhabditis elegans High-affinity Choline Transporter is Expressed in Cholinergic Neurons and is Required for Normal Cholinergic Function.  Presented at the Neruonal Synaptic Function and Behavior Caenorhabditis elegans Topic Meeting 2.  Madison, Wisconsin.

Frisby, D., Walker III, W., Rand, J.  2006.  Isolation of Caenorhabditis elegans Mutants Defective in Regulating Cholinergic Gene Expression. Presented at the Neruonal Synaptic Function and Behavior Caenorhabditis elegans Topic Meeting 2.  Madison, Wisconsin.